TWiV 365: Blood, feuds, and a foodborne disease

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

For a TWiV Thanksgiving, Vincent, Alan, and Kathy trace the feud over genome editing, a new virus discovered in human blood, and the origins of hepatitis A virus.

• Battle over genome editing (Wired) 10:05


• First description of CRISPR in E. coli (J Bacteriol) 14:00


• Cas genes identified (Mol Micro) 14:20


• CRISPR-Cas provides immunity to bacterial viruses (Science) 15:35


• Cas9-crRNA complex mediates DNA cleavage (PNAS) 16:55


• Dual RNA-guided DNA endonuclease in bacterial immunity (Science) 17:50


• Genome engineering using CRISPR/Cas (Science) 18:50


• Cpf1 endonuclease (Cell) 23:10


• Classification of CRISPR/Cas systems (Nat Rev Micro) 23:50


• Summit on gene editing 21:25


• Novel human virus in transfusion recipients (mBio) 27:00


• Origins of hepatitis A virus in small mammals (PNAS) 55:05


• Image credit


• Letters read on TWiV 365 7:00, 1:20:35

This episode is sponsored by ASM Microbe, ASM Biodefense, and the 32nd Clinical Virology Symposium. (1:10, 1:18:30)

Kathy - Tardigrade genome sequence (video)Alan - XKCD on Gates polio eradication siteVincent - Lifting the Impenetrable Veil by Charles Calisher

Judi - Jennifer Doudna's Ted TalkRamon - Map of the Sky

Send your virology questions and comments to [email protected]